Evaluating Skin Protein Expression

The skin sections were stained with the following monoclonal antimouse antibodies: MMP-1, MMP-2, IL-6, COX-2, NF-κB, Nrf-2, aquaporin, and filaggrin (Genetex, Beverly, MA, USA). The skin sections were examined under a microscope, and the protein expression was determined using Image J software, as described previously (National Institutes of Health, Bethesda, MD, USA) [38 (link)].

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Wu P.Y., Lyu J.L., Liu Y.J., Chien T.Y., Hsu H.C., Wen K.C, & Chiang H.M. (2017). Fisetin Regulates Nrf2 Expression and the Inflammation-Related Signaling Pathway to Prevent UVB-Induced Skin Damage in Hairless Mice. International Journal of Molecular Sciences, 18(10), 2118.

Publication 2017

MMP-1 IL-6 COX-2 NF-κB

Aquaporin Cox 2 Filaggrin Microscope Mmp 1 Mmp 2 Monoclonal antibodies Nf κb Nrf 2 Protein Skin

Corresponding Organization : China Medical University

Other organizations : China Medical University Hospital

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Variable analysis

independent variables

Monoclonal antimouse antibodies: MMP-1, MMP-2, IL-6, COX-2, NF-κB, Nrf-2, aquaporin, and filaggrin

dependent variables

Protein expression

control variables

Control variables not explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the provided information.

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