Flow Cytometric Analysis of Liver Immune Cells

Mice were sacrificed and the liver was harvested for analysis ex vivo as previously described (Park et al., 2017 (link)). Cells were isolated and stained with anti-mouse CD45 (1:100 dilution, Biolegend, #103116), anti-mouse F4/80 (1:100 dilution, Biolegend, #123108), anti-mouse CD206 (1:100 dilution, Biolegend, #141720), and anti-mouse CD11c (1:100 dilution, Biolegend, #117310), and then tested using flow cytometry (LSRFortessaTM cell analyzer, BD, United States). Data were analyzed with FlowJo V10.6.

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Zhou X., Yi L., Lang H., Zhang J., Zhang Q., Yu L., Zhu J, & Mi M. (2022). Dihydromyricetin-Encapsulated Liposomes Inhibit Exhaustive Exercise-Induced Liver Inflammation by Orchestrating M1/M2 Macrophage Polarization. Frontiers in Pharmacology, 13, 887263.

Publication 2022

anti-mouse CD45 anti-mouse F4/80 anti-mouse CD206 anti-mouse CD11c LSRFortessaTM cell analyzer

Cell Dilution Flow cytometry Liver Mouse

Corresponding Organization :

Other organizations : China National Center for Food Safety Risk Assessment

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Variable analysis

independent variables

Treatment (sacrificing mice and harvesting liver)

dependent variables

Levels of CD45, F4/80, CD206, and CD11c on isolated cells, as measured by flow cytometry

control variables

Mice used for the experiment
Procedures for cell isolation and staining, as previously described (Park et al., 2017)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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