Unilateral hindlimb ischemia (HLI) was induced on 9-week-old female Swiss mice (Janvier Labs, n = 8) after femoral artery excision under 2% isoflurane anesthesia as previously described [40 (link)]. LASER Doppler perfusion imaging (Perimed, Craponne, France) was used to assess revascularization from day 0 to day 21 after surgery. Perfusion measurements were expressed as an ischemic-to-contralateral ratio of hind limb blood flow normalized to the day of surgery.
Louis B., Nail V., Nachar O., Bouhlel A., Moyon A., Balasse L., Simoncini S., Chabert A., Fernandez S., Brige P., Hache G., Tintaru A., Morgat C., Dignat-George F., Garrigue P, & Guillet B. (2023). Design and preclinical evaluation of a novel apelin-based PET radiotracer targeting APJ receptor for molecular imaging of angiogenesis. Angiogenesis, 26(3), 463-475.
Other organizations :
Unité de recherche sur les maladies cardiovasculaires et métaboliques, Aix-Marseille Université, Hôpital de la Timone, Hôpital Nord, Centre Interdisciplinaire de Nanoscience de Marseille, Centre National de la Recherche Scientifique, Institut de Neurosciences Cognitives et Intégratives d’Aquitaine, Centre Hospitalier Universitaire de Bordeaux
Unilateral hindlimb ischemia (HLI) induced on 9-week-old female Swiss mice (Janvier Labs, n = 8) after femoral artery excision under 2% isoflurane anesthesia
dependent variables
Revascularization assessed by LASER Doppler perfusion imaging (Perimed, Craponne, France) from day 0 to day 21 after surgery
Perfusion measurements expressed as an ischemic-to-contralateral ratio of hind limb blood flow normalized to the day of surgery
control variables
Female Swiss mice (Janvier Labs, n = 8)
Age of mice: 9 weeks
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