The pGL4.32 and pGL4.75 were extracted separately according to the instructions and the transfection solution was prepared according to the requirements at the time of use. Cells in good growth condition were seeded in 96-well plates at a density of 3 × 105 cells/mL. 0.1, 1, 10 μM polydatin and 10–2 μM Dexamethasone in TNF-α medium, and then the reporter gene activity was tested according to the procedure provided by the reagent company.
Protocol detail
Investigating Polydatin and Dexamethasone Effects
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : Tianjin University of Traditional Chinese Medicine
Top 5 similar protocols
Variable analysis
independent variables
- Polydatin concentrations (0.1, 1, 10 μM)
- Dexamethasone concentration (10^-2 μM)
- Reporter gene activity
- Cells in good growth condition
- Cell seeding density (3 × 10^5 cells/mL)
- TNF-α medium
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!