Palmitate Uptake and Oxidation Assay

HAOEC were submitted to shear stress or left under static condition in complete MV2 medium with 100 μM unlabeled palmitate and 50 μM carnitine. Cells were then incubated for 2 h in complete medium containing 2 μCi/mL [9,10 ³H] palmitate (Perkin Elmer, France). Medium was transferred into glass vial and [³H]-H₂O was captured on a piece of Whatman paper soaked with H₂O over a period of 48 h at 37°C. Radioactivity was measured by liquid scintillation counting (Schoors et al., 2015 (link); Kalucka et al., 2018 (link)).

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Bousquet D., Nader E., Connes P, & Guillot N. (2024). Liver X receptor agonist upregulates LPCAT3 in human aortic endothelial cells. Frontiers in Physiology, 15, 1388404.

Publication 2024

[9,10 3H] palmitate

Corresponding Organization :

Other organizations : Université Claude Bernard Lyon 1

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Variable analysis

independent variables

Shear stress
Static condition

dependent variables

[3H]-H2O production

control variables

Complete MV2 medium
100 μM unlabeled palmitate
50 μM carnitine
2 μCi/mL [9,10 3H] palmitate

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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