Histological Analysis of Intestinal Tight Junctions

The paraffin-embedded tissues were sectioned into 3-μm-thick slides. According to standard histological protocols, hematoxylin and eosin (HE) staining was conducted. For immunohistochemistry (IHC), the following primary antibodies were used to incubate the slides, respectively, after blocking: bacterial lipopolysaccharide (LPS; HycultBiotech #HM6011, Netherlands), lipoteichoic acid (LTA; Santa Cruz, #sc-57752, United States), occludin (Servicebio #GB111401, China), and zonula occludens 1 (ZO-1; Servicebio #GB111981, China). IHC quantification involved the calculation of positive areas (occludin and ZO-1) using Aipathwell software (Version 2.0, Servicebio, China).

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Fan H., Wang Y., Han M., Wang L., Li X., Kuang X., Du J, & Peng F. (2024). Multi-omics-based investigation of Bifidobacterium’s inhibitory effect on glioma: regulation of tumor and gut microbiota, and MEK/ERK cascade. Frontiers in Microbiology, 15, 1344284.

Publication 2024

GB111401 GB111981 Aipathwell software

Corresponding Organization :

Other organizations : Sichuan University

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Variable analysis

independent variables

Bacterial lipopolysaccharide (LPS)
Lipoteichoic acid (LTA)
Occludin
Zonula occludens 1 (ZO-1)

dependent variables

Positive areas (occludin and ZO-1)

control variables

Paraffin-embedded tissue sections with 3-μm thickness
Hematoxylin and eosin (HE) staining

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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