Zur-DNA Binding Electrophoresis Assay

Wild-type or mutant Zur proteins were mixed with a fixed amount (65 nM) of different DNA probes (PznuA site 1, PzitB) in 20 μl of reaction buffer (20 mM Tris–HCl, pH 7.8, 150 mM NaCl, 5 mM DTT, 0.1 mg/ml BSA, 5% glycerol and 0.1 μg of poly(dI-dC)) as described previously (21 (link)). After electrophoresis, gels were visualized by using Amersham Typhoon RGB Biomolecular Imager with Cy2 filters.

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Choi Y., Koh J., Cha S.S, & Roe J.H. (2024). Activation of zinc uptake regulator by zinc binding to three regulatory sites. Nucleic Acids Research, 52(8), 4185-4197.

Publication 2024

Typhoon RGB Biomolecular Imager

Corresponding Organization : Seoul National University

Other organizations : Ewha Womans University

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Variable analysis

independent variables

Wild-type or mutant Zur proteins

dependent variables

Binding of Zur proteins to DNA probes (PznuA site 1, PzitB)

control variables

Fixed amount (65 nM) of different DNA probes
Reaction buffer (20 mM Tris–HCl, pH 7.8, 150 mM NaCl, 5 mM DTT, 0.1 mg/ml BSA, 5% glycerol and 0.1 μg of poly(dI-dC))

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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