Quantitative PCR and Scanning Electron Microscopy

Quantitative PCR using specific primers (Table S1) and statistical analyses using the Graph Pad Prism 8 (GraphPad Software, San Diego, CA, USA) were performed as described in a previous report [37 (link)]. Scanning electron microscopy (SEM) using a HITACHI S-4300 microscope was operated at 5 keV (the detector features 1280 × 960 pixel) as basically reported in our previous study [37 (link)]. For estimation of the statistical difference between study groups, the Student’s t-test for two group comparison or one-way ANOVA followed by a Tukey’s multiple comparison test were used.

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Nishikiori N., Takada K., Sato T., Miyamoto S., Watanabe M., Hirakawa Y., Sekiguchi S., Furuhashi M., Yorozu A., Takano K., Miyazaki A., Suzuki H, & Ohguro H. (2023). Physical Properties and Cellular Metabolic Characteristics of 3D Spheroids Are Possible Definitive Indices for the Biological Nature of Cancer-Associated Fibroblasts. Cells, 12(17), 2160.

Publication 2023

Graph Pad Prism 8 S-4300 microscope

Anova Microscope Primers Prism Scanning electron microscopy Student

Corresponding Organization : Sapporo Medical University

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Variable analysis

independent variables

Experimental conditions or treatments that were manipulated by the researchers

dependent variables

Gene expression levels measured by quantitative PCR
Cellular morphology observed by scanning electron microscopy (SEM)

control variables

Not explicitly mentioned

controls

Positive and negative controls were not explicitly mentioned

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