Protocol detail

Quantitative Transcriptomic Analysis of Soybean

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Plant samples stored at −80 °C were run according to the method provided in the Plant Total RNA Extraction Kit (TIANGEN). qRT-PCR was performed using the experimental method provided by the PrimeScript^TM RT Kit (Takara, Shiga, Japan). The primers were designed by Primer Premier 5.0, in which the soybean actin gene was used as a control. An ABI Prism 7500 real-time PCR system (Applied Biosystems, Foster City, CA, USA) was used to perform qRT-PCR [26 (link)]. The resulting data was analyzed using the 2^−ΔΔCT method [45 (link)].

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Wang D., Liu Y.X., Yu Q., Zhao S.P., Zhao J.Y., Ru J.N., Cao X.Y., Fang Z.W., Chen J., Zhou Y.B., Chen M., Ma Y.Z., Xu Z.S, & Lan J.H. (2019). Functional Analysis of the Soybean GmCDPK3 Gene Responding to Drought and Salt Stresses. International Journal of Molecular Sciences, 20(23), 5909.

Publication 2019

Plant Total RNA Extraction Kit PrimeScriptTM RT Kit ABI Prism 7500

Actin Gene Plant Plant rna Primer Prism Soybean

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Gene expression levels measured by qRT-PCR

control variables

Plant samples stored at -80°C
Soybean actin gene used as a control
ABI Prism 7500 real-time PCR system used to perform qRT-PCR

controls

Positive control: Soybean actin gene
Negative control: Not explicitly mentioned

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