PLGA (lactic acid: glycolide 65:35, molecular weight (Mw) = 40,000–75,000), chitosan (Mw = 50,000–190,000 Da), poly vinyl alcohol (PVA, Mw = 31,000–50,000), concanavalin A, and complete Freund’s adjuvant were purchased from Sigma-Aldrich (St. Louis, MO, USA).
We purchased the Sodium tripolyphosphate (Mw = 367.86) from Aladdin and Enhanced Cell Counting Kit-8 from Beyotime. Likewise, the BCA Protein Assay Kit was acquired from CW Biotech, China.
The Roswell Park Memorial Institute medium (RPMI 1640), heat-inactivated fetal bovine serum (FBS), and penicillin–streptomycin solutions were purchased Gibco. Antibodies PE rat anti-mouse CD86 (clone: GL1), PE rat anti-mouse CD83 (clone: Michel-19), APC hamster anti-mouse CD11c (clone: HL3), APC hamster anti-mouse CD3e (clone: 145-2C11), FITC rat anti-mouse CD4 (clone: RM4-5), and FITC rat anti-mouse CD8α (clone: 53-6.7) were purchased from Bio-legend. The purified recombinant proteins of HcARF1 and pET-32a expressed in E. coli [5 (link)] were obtained from Molecular Parasitology and Immunology laboratory at Nanjing Agricultural University.
We purchased the Sodium tripolyphosphate (Mw = 367.86) from Aladdin and Enhanced Cell Counting Kit-8 from Beyotime. Likewise, the BCA Protein Assay Kit was acquired from CW Biotech, China.
The Roswell Park Memorial Institute medium (RPMI 1640), heat-inactivated fetal bovine serum (FBS), and penicillin–streptomycin solutions were purchased Gibco. Antibodies PE rat anti-mouse CD86 (clone: GL1), PE rat anti-mouse CD83 (clone: Michel-19), APC hamster anti-mouse CD11c (clone: HL3), APC hamster anti-mouse CD3e (clone: 145-2C11), FITC rat anti-mouse CD4 (clone: RM4-5), and FITC rat anti-mouse CD8α (clone: 53-6.7) were purchased from Bio-legend. The purified recombinant proteins of HcARF1 and pET-32a expressed in E. coli [5 (link)] were obtained from Molecular Parasitology and Immunology laboratory at Nanjing Agricultural University.
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