Genotyping APOE Exon 4 Variants

The variants in exon 4 in APOE (GenBank: NM_000041)—that is, c.388T>C and c.526C>T—were analyzed by polymerase chain reaction and Sanger sequencing in DNA extracted from peripheral leukocytes. Primers were designed using Primer3Plus (Bioinformatics, Arlington, VA, USA) [40 (link)] and the polymerase chain reaction products were purified and Sanger-sequenced using an ABI 3730xl DNA analyzer and ABI BigDye terminator cycle-sequencing kits v3.1 (Thermo Fisher Scientific, Waltham, MA, USA). Sequences were analyzed with the DNA Sequencing Analysis software program (version 5.1; Applied Biosystems, Foster City, CA, USA) and the SeqScape software program (version 2.7; Thermo Fisher Scientific).

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Hellstrøm T., Andelic N., de Lange A.M., Helseth E., Eiklid K, & Westlye L.T. (2021). Apolipoprotein ɛ4 Status and Brain Structure 12 Months after Mild Traumatic Injury: Brain Age Prediction Using Brain Morphometry and Diffusion Tensor Imaging. Journal of Clinical Medicine, 10(3), 418.

Publication 2021

ABI 3730xl DNA analyzer DNA Sequencing Analysis software program SeqScape software program

Abi 1 Apoe Dna sequencing analysis Exon Leukocytes Polymerase chain reaction Primers

Corresponding Organization : Oslo University Hospital

Other organizations : University of Oslo, University of Lausanne, University of Oxford

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Variable analysis

independent variables

Variants in exon 4 in APOE (GenBank: NM_000041), specifically c.388T>C and c.526C>T

dependent variables

Presence and/or frequency of the APOE variants c.388T>C and c.526C>T

control variables

DNA extracted from peripheral leukocytes
Primers designed using Primer3Plus
Polymerase chain reaction products purified and Sanger-sequenced using an ABI 3730xl DNA analyzer and ABI BigDye terminator cycle-sequencing kits v3.1
Sequences analyzed with the DNA Sequencing Analysis software program (version 5.1) and the SeqScape software program (version 2.7)

controls

No positive or negative controls were explicitly mentioned in the provided information.

Annotations

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