Corticosterone Measurement in Cancer-Induced Anesthesia

At the time of euthanasia, the animals were anesthetized by intraperitoneal injection, with a mixture of medetomidine, midazolam, and butorphanol. Blood samples were collected from the axillary artery from 10:00 to 11:00 a.m. on day 49 after cancer-cell inoculation. The serum was obtained by centrifugation of the blood sample at 3000 rpm for 15 min at 4 °C. The corticosterone level in the serum was measured using an enzyme-linked immunosorbent assay kit (Assaypro, St. Charles, MO, USA), following the manufacturer’s instructions [24 (link)].

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Zhang J.H., Wang K.Y., Kubo K.Y, & Azuma K. (2022). Chewing Behavior Attenuates Lung-Metastasis-Promoting Effects of Chronic Stress in Breast-Cancer Lung-Metastasis Model Mice. Cancers, 14(23), 5950.

Publication 2022

enzyme-linked immunosorbent assay kit

Animals Axillary artery Blood Butorphanol Cancer inoculation Cell Centrifugation Corticosterone Enzyme linked immunosorbent assay Euthanasia Intraperitoneal injection Medetomidine Midazolam Serum

Corresponding Organization : University of Occupational and Environmental Health Japan

Other organizations : Nagoya Women's University

Top 5 similar protocols

Variable analysis

independent variables

Anesthetization method (intraperitoneal injection of medetomidine, midazolam, and butorphanol)

dependent variables

Corticosterone level in serum

control variables

Time of blood sample collection (10:00 to 11:00 a.m. on day 49 after cancer-cell inoculation)
Blood sample processing (centrifugation at 3000 rpm for 15 min at 4 °C)

controls

Positive control: None specified
Negative control: None specified

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