Purification and Labeling of Anti-hVLDLR Antibody

Mouse anti-hVLDLR monoclonal antibody mAb 5F3^{28 (link)} was purified as described
earlier.^{29 (link)} Goat
secondary anti-mouse polyclonal antibodies conjugated with HRP and HRP substrate
SureBlue TMB were purchased from KPL (Gaithersburg, MD). Thrombin CleanCleave
kit was obtained from Sigma (St. Louis, MO), Blocker BSA in TBS,
sulfo-NHS-acetate, and p-hydroxyphenylglyoxal were from Thermo Scientific
(Rockford, IL).

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Yakovlev S, & Medved L. (2017). Interaction of Fibrin with the Very Low Density Lipoprotein Receptor: Further Characterization and Localization of the VLDL Receptor-Binding Site in Fibrin βN-Domains. Biochemistry, 56(19), 2518-2528.

Publication 2017

Thrombin CleanCleavekit Blocker BSA in TBS sulfo-NHS-acetate p-hydroxyphenylglyoxal

Anti antibodies Anti antibody Mouse Sulfo nhs acetate Thrombin

Corresponding Organization :

Other organizations : University of Maryland, Baltimore

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Variable analysis

independent variables

Mouse anti-hVLDLR monoclonal antibody mAb 5F3

dependent variables

Not explicitly mentioned

control variables

Goat secondary anti-mouse polyclonal antibodies conjugated with HRP
HRP substrate SureBlue TMB
Thrombin CleanCleave kit
Blocker BSA in TBS
Sulfo-NHS-acetate
P-hydroxyphenylglyoxal

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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