Purification of Murine β-NGF from Mouse Glands

Murine β-NGF from adult male mouse submaxillary glands was purified in our laboratory as already described (Bocchini and Angeletti, 1969 (link)), with minor revisions. Male Crl:CD1(ICR) mice aged 6–8 months were obtained from Charles River Italia (Calco, Varese, Italy) and sacrificed by cervical translocation. The anti-NGF antibody was raised in goat and purified following an established procedure (Stoeckel and Thoenen, 1975 (link); Stoeckel et al., 1976 (link)).

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Baldassarro V.A., Cescatti M., Rocco M.L., Aloe L., Lorenzini L., Giardino L, & Calzà L. (2023). Nerve growth factor promotes differentiation and protects the oligodendrocyte precursor cells from in vitro hypoxia/ischemia. Frontiers in Neuroscience, 17, 1111170.

Publication 2023

Adult Anti antibody Cervical Goat Icr mice Male Mouse Murine River Submaxillary glands Translocation

Corresponding Organization : University of Bologna

Other organizations : Fondazione IRET ONLUS

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Variable analysis

independent variables

Murine β-NGF from adult male mouse submaxillary glands

dependent variables

Purification of Murine β-NGF

control variables

Male Crl:CD1(ICR) mice aged 6–8 months obtained from Charles River Italia (Calco, Varese, Italy)

controls

Positive control: Anti-NGF antibody raised in goat and purified following an established procedure (Stoeckel and Thoenen, 1975; Stoeckel et al., 1976)
Negative control: Not explicitly mentioned

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