Systolic BP in Gch1 Knockout Mice

Systolic blood pressure in conscious wild-type and Gch1^fl/flTie2cre mice was determined using the VisitechR computerized tail-cuff plethysmography system (Visitech) following 5 days of training and 3 days baseline periods. Experiments were performed between the hours of 8:00 and 12:00 am. The animal tails were passed through a cylindrical latex tail-cuff and taped down to reduce movement. Twenty readings were taken per mouse of which the first five readings were discarded. The remaining 15 readings were used to calculate the mean systolic blood pressure in each mouse.