Theileria annulata-infected cells TaNM1 were cultured in 12-well culture plates at 105 cells per well and treated with 200 ng/ml (stock 2 mg/ml in ethanol) buparvaquone (BW720c, Sigma). Meanwhile, cells were treated with the same amount of ethanol that used for dissolve BW720c as the control group. HeLa cells were also treated with the same concentration of BW720c to test its effect on the protein level of telomerase reverse transcriptase.
5 × 105 TaNM1 cells were treated with 30 nmol/ml BIBR, 300 nmol/ml novobiocin, and 1 nmol/ml Geldanamycin (MedChemExpress), respectively. The equal volume of DMSO was used to treat the cells as control group.
5 × 105 TaNM1 cells were treated with 30 nmol/ml BIBR, 300 nmol/ml novobiocin, and 1 nmol/ml Geldanamycin (MedChemExpress), respectively. The equal volume of DMSO was used to treat the cells as control group.
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