Participants’ fasting, cryopreserved plasma samples were shipped to Temple University (Philadelphia, PA) for centralized quantification of levels of immune/inflammatory biomarkers using enzyme-linked immunosorbent assay kits: soluble CD14 (sCD14; R&D), soluble CD163 (sCD163; IQ Products), MCP-1 (R&D), high-sensitivity IL-6 (R&D), Lp-PLA2 (R&D), and oxLDL (Mercodia) [7 (link)]. D-dimer was measured using the HemosIL D-dimer HS 500 kit on the ACL TOP (Werfen). Fasting, cryopreserved serum samples were shipped to Quest Diagnostics for quantification of high-sensitivity C-reactive protein (hs-CRP) by commercially available assays.
Zanni M.V., Foldyna B., McCallum S., Burdo T.H., Looby S.E., Fitch K.V., Fulda E.S., Autissier P., Bloomfield G.S., Malvestutto C.D., Fichtenbaum C.J., Overton E.T., Aberg J.A., Erlandson K.M., Campbell T.B., Ellsworth G.B., Sheth A.N., Taiwo B., Currier J.S., Hoffmann U., Lu M.T., Douglas P.S., Ribaudo H.J, & Grinspoon S.K. (2022). Sex Differences in Subclinical Atherosclerosis and Systemic Immune Activation/Inflammation Among People With Human Immunodeficiency Virus in the United States. Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America, 76(2), 323-334.
Assays Cd163 D dimer Diagnostics Enzyme linked immunosorbent assay High sensitivity c reactive protein Inflammatory Lp pla2 Mcp 1 OxldlPlasma Scd14 SensitivitySerum
Corresponding Organization : Harvard University
Other organizations :
Temple University, Boston College, Clinical Research Institute, Duke University, The Ohio State University Wexner Medical Center, University of Cincinnati Medical Center, University of Alabama at Birmingham, Icahn School of Medicine at Mount Sinai, University of Colorado Anschutz Medical Campus, Cornell University, Emory University, Northwestern University, Center for Global Health, University of California, Los Angeles, Cancer Research And Biostatistics
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