Immunohistochemical Analysis of Neurodegenerative Markers

The immunohistochemical study was performed as previously described [57 (link),58 (link)]. Primary antibodies used were: anti-GFAP (Santa Cruz Biotechnology, 1:100 in PBS, v/v), anti-Iba1 (Santa Cruz Biotechnology, 1:100 in PBS, v/v), anti-TH (Millipore, 1:500 in PBS, v/v, Burlington, MA, USA), anti-DAT (Santa Cruz Biotechnology, 1:300 in PBS, v/v), anti-α-syn (Santa Cruz Biotechnology, 1:100 in PBS, v/v). The slices were then rinsed with PBS and treated with secondary antibody the next day. A biotin-conjugated goat anti-rabbit IgG and an avidin-biotin peroxidase complex were used to identify specific labeling (Vector). Five stained sections from each mouse were scored blindly and examined using a Leica DM6 microscope (Leica Microsystems SpA, Milan, Italy) in accordance with standard procedures [59 (link),60 (link)]. The histogram profile is related to the positive pixel intensity value obtained.

Free full text: Click here

Cordaro M., D’Amico R., Fusco R., Genovese T., Peritore A.F., Gugliandolo E., Crupi R., Di Paola D., Interdonato L., Impellizzeri D., Cuzzocrea S., Di Paola R, & Siracusa R. (2022). Actaea racemosa L. Rhizome Protect against MPTP-Induced Neurotoxicity in Mice by Modulating Oxidative Stress and Neuroinflammation. Antioxidants, 12(1), 40.

Publication 2022

anti-GFAP anti-Iba1 anti-TH anti-DAT anti-α-syn Leica DM6 microscope

Anti igg Anti syn Antibodies Antibody Avidin Biotin Gfap Goat Microscope Mouse Peroxidase Rabbit Vector

Corresponding Organization : Saint Louis University

Top 5 similar protocols

Variable analysis

independent variables

Primary antibodies used: anti-GFAP, anti-Iba1, anti-TH, anti-DAT, anti-α-syn

dependent variables

Positive pixel intensity value obtained

control variables

The slices were rinsed with PBS and treated with secondary antibody the next day
A biotin-conjugated goat anti-rabbit IgG and an avidin-biotin peroxidase complex were used to identify specific labeling
Five stained sections from each mouse were scored blindly and examined using a Leica DM6 microscope in accordance with standard procedures

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!