Custom SNP Microarray for Genome Profiling

A custom SNP microarray designed in our previous studies was used to detect the genomic alterations in diploid strains [31 (link),43 (link)]. In brief, the control DNA extracted from the heterozygous strain JSC24-2 was labeled with Cy3-dUTP, and the experimental DNA was labeled with Cy5-dUTP. The labeled DNA was mixed and competitively hybridized at 62°C. A GenePix scanner and GenePix Pro-6.1 software were used to examine the ratio of hybridization of the control and experimental DNA. The designs of the array are on the Gene Expression Omnibus (GEO) dataset, with the accession number of GPL20144.