Plant Ethylene-Response Assays and Analyses

Details for plant growth and ethylene-response assay, gene expression analysis, protein fractionation, membrane-based Y2H assay, Co-IP and BiFC assays, and ubiquitination analysis are described in SI Appendix, SI Materials and Methods. The primers used in this study are listed in SI Appendix, Table S1.

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Ma B., Zhou Y., Chen H., He S.J., Huang Y.H., Zhao H., Lu X., Zhang W.K., Pang J.H., Chen S.Y, & Zhang J.S. (2018). Membrane protein MHZ3 stabilizes OsEIN2 in rice by interacting with its Nramp-like domain. Proceedings of the National Academy of Sciences of the United States of America, 115(10), 2520-2525.

Publication 2018

Assays Ethylene Fractionation Gene expression analysis Membrane Plant growth Primers Protein Ubiquitination

Corresponding Organization :

Other organizations : State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences

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Variable analysis

independent variables

Plant growth and ethylene-response assay
Gene expression analysis
Protein fractionation
Membrane-based Y2H assay
Co-IP and BiFC assays
Ubiquitination analysis

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive controls
Negative controls

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