TW samples were analyzed by USGS using 12 target organic (482 unique analytes) and 1 inorganic (19 analytes) methods (Table S1).63 (link) Analyses conducted at 3 USGS laboratories included the (1) National Water Quality Laboratory (NWQL) in Denver, Colorado (volatile organic compounds (VOC);64 (link) steroid hormones and related compounds;65 organic wastewater indicator (OWI) compounds;66 (link) human-use pharmaceuticals, pharmaceutical metabolites, and polar organic compounds;67 (link) and trace elements68 ,69 ); (2) Organic Geochemistry Research Laboratory (OGRL) in Lawrence, Kansas (acetamide herbicides and degradation products;70 glyphosate, glufosinate, and aminomethylphosphonic acid;71 steroid hormones and phytoestrogens;72 (link) triazine and phenylurea herbicides;73 and antibiotics74 ); and (3) Organic Chemistry Research Laboratory (OCRL) in Sacramento, California (pesticide and pesticide degradates;75 (link) diuron, diuron degradates, and neonicotinoid insecticides; 76 and DBP77 (link)). Per/polyfluoroalkyl substances (PFAS) were analyzed78 (link)−80 at the EPA, Colorado School of Mines (CSM), and NWQL. Pharmaceutical samples were syringe filtered (0.7 μm nominal pore size, glass fiber) in the field. Pharmaceutical and VOC bottles were pretreated with ascorbic acid to neutralize chlorine/chloramine. In vitro estrogen (ER), androgen (AR), and glucocorticoid (GR) activities were assessed by EPA34 (link),55 (link) and USGS.34 (link),55 (link),81 (link) Results are in Tables S2−S5 and at Romanok et al.82 (link)