Comprehensive RNA Sequencing of C. oleifera

Four tissues of C. oleifera, including tender shoots, young leaves, flower buds and flowers, were harvested in 2010 from East Park of Kunming Botanic Garden, Yunnan Province, China. All necessary permits were obtained from Wei-bang Sun, who is the director of the Kunming Botanical Garden, the Chinese Academy of Sciences. All samples were flash frozen in liquid nitrogen and stored at −80°C for RNA extraction. Total RNA was extracted by a modified CTAB method. The quality and quantity of total RNA were analyzed using agarose gel electrophoresis and Agilent 2100 Bioanalyzer RNA chip (Agilent Technologies, CA). cDNA library construction and normalization were performed as described previously [44] (link). All libraries were combined into a single pool and sequenced using the 454 GS-FLX platform (Roche, IN, USA).