Gateway Cloning of Plasmids

The plasmids pT3-IRES-GFP, pT3-FAK-IRES-GFP, pT3-FAK (K454R)-IRES-GFP, pT3-CAT, and pT3-MET were generated by Gateway cloning as described.^{(9 (link),10 (link))} The plasmids were purified using GeneJET Plasmid Maxiprep Kit (Thermo Fisher Scientific) for hydrodynamic tail vein injection.

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Shang N., Wang H., Bank T., Perera A., Joyce C., Kuffel G., Zilliox M.J., Cotler S.J., Ding X., Dhanarajan A., Breslin P, & Qiu W. (2019). FAK and β-Catenin Cooperate to Induce Hepatocellular Carcinoma. Hepatology (Baltimore, Md.), 70(5), 1631-1645.

Publication 2019

GeneJET Plasmid Maxiprep Kit

Hydrodynamic Ires Plasmid Tail Vein

Corresponding Organization : Loyola University Chicago

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Variable analysis

independent variables

PT3-IRES-GFP
PT3-FAK-IRES-GFP
PT3-FAK (K454R)-IRES-GFP
PT3-CAT
PT3-MET

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Not specified
Not specified

Annotations

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