Antibody-based detection of nuclear and synaptic proteins

The primary antibodies used were rabbit polyclonal LAP1 [11] (link); rabbit polyclonal lamin B1 (Santa Cruz Biotechnology); mouse monoclonal β-tubulin (Invitrogen); mouse monoclonal synaptophysin (Synaptic Systems); rabbit polyclonal CBC3C that recognizes the C-terminal of PP1γ [16] (link); Myc-tag antibody (Cell Signaling), that recognizes Myc-fusion proteins; and HA-tag antibody (Clontech), that recognizes HA-fusion proteins. The secondary antibodies used were anti-mouse and anti-rabbit horseradish peroxidase-linked antibodies (GE Healthcare) for ECL detection.

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Santos M., Domingues S.C., Costa P., Muller T., Galozzi S., Marcus K., da Cruz e Silva E.F., da Cruz e Silva O.A, & Rebelo S. (2014). Identification of a Novel Human LAP1 Isoform That Is Regulated by Protein Phosphorylation. PLoS ONE, 9(12), e113732.

Publication 2014

lamin B1 mouse monoclonal β-tubulin Myc-tag antibody

Anti antibodies Antibodies Antibody Horseradish peroxidase Lamin b1 Lap1 Mouse Proteins Rabbit Synaptophysin Tubulin

Corresponding Organization : Ruhr University Bochum

Top 5 similar protocols

Variable analysis

independent variables

Rabbit polyclonal LAP1
Rabbit polyclonal lamin B1
Mouse monoclonal β-tubulin
Mouse monoclonal synaptophysin
Rabbit polyclonal CBC3C that recognizes the C-terminal of PP1γ
Myc-tag antibody
HA-tag antibody

dependent variables

Not explicitly mentioned

control variables

Anti-mouse horseradish peroxidase-linked antibodies
Anti-rabbit horseradish peroxidase-linked antibodies

positive controls

Rabbit polyclonal LAP1
Rabbit polyclonal lamin B1
Rabbit polyclonal CBC3C that recognizes the C-terminal of PP1γ

negative controls

Mouse monoclonal β-tubulin
Mouse monoclonal synaptophysin
Myc-tag antibody
HA-tag antibody

Annotations

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