Female BALB/c nude mice (5–6 weeks old, 20 g) were purchased from ORIENT (Gapyeong, South Korea). The mice were grouped as follows (n = 3 mice per group): (1) PBS as a negative control, (2) NEs, (3) liposomes, and (4) NE-liposomes. The liposomal formulations were injected intravenously (i.v.) by a single treatment, the same as the therapeutic dose. To determine the biochemical toxicity of the liposomal formulations, the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and blood urea nitrogen (BUN) were analyzed using diagnostic kits (Roche, Berlin, Germany).27 (link) Blood samples were collected from the mice through the retro-orbital sinus on days 1 and 7 after the injection of liposomes. Serum was obtained from the blood by centrifugation at 3000 rpm at 4 °C for 10 min (Smart R17 Plus, Hanil Scientific Inc., Seoul, South Korea).
Protocol detail
Toxicity Assessment of Liposomal Formulations
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Alanine aminotransferase
Aspartate aminotransferase
Balb c mice
Blood
Blood urea nitrogen
Centrifugation
Diagnostic
Female
Liposomes
Mice
Nude
Orient
Serum
Sinus
Therapeutic
Corresponding Organization :
Other organizations : Konkuk University, Samsung Medical Center, Sungkyunkwan University, Korea Research Institute of Chemical Technology
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Variable analysis
independent variables
- Liposomal formulations (PBS, NEs, liposomes, and NE-liposomes)
- Levels of aspartate aminotransferase (AST)
- Levels of alanine aminotransferase (ALT)
- Levels of blood urea nitrogen (BUN)
- Female BALB/c nude mice (5–6 weeks old, 20 g)
- Single intravenous (i.v.) treatment of liposomal formulations at the same therapeutic dose
- Negative control: PBS
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