Paraffin-embedded sections of tumor tissues were stained for PTPN14 and PTEN expression. Immunohistochemistry (IHC) and the scoring system for PTPN14 (1:200, SAB2700311, Sigma-Aldrich, USA) and PTEN (1:100, ab31392, Abcam, UK) were performed as we previously described [50 (link)].
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