Serum levels of mature BDNF, proBDNF, and MMP-9 were measured using the human BDNF ELISA Kit (Adipo Bioscience, Santa Clara, CA, USA), the human proBDNF ELISA Kit (Adipo Bioscience), and the human MMP-9 ELISA Kit (R&D Systems, Minneapolis, MN, USA), respectively. The optical density of each well was measured using an automated microplate reader (Emax; Molecular Devices, Sunnyvale, CA, USA) [21 (link)].
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