SARS-CoV-2 Infection Dynamics in K18-ACE2 Mice

The Indiana University School approved all animal studies of the Institutional Animal Care and Use Committee (IACUC) and the Indiana University Institutional Biosafety Committee (IBC). Cg-Tg(K18-ACE2)2Prlmn/J mice (Jackson Laboratory, Bar Harbor, ME, Stock No: 034860) were used in this study. At approximately 8 weeks of age, K18-ACE2 mice were either mock infected (media only) or were infected (media containing virus) intranasally (20μl) with SARS-CoV-2 isolate USA-WA1/2020 (BEI Resources, Manassas, VA), n=4-5/group using methods described previously ^{10 (link),11 (link)}. Mice were administered with a viral concentration of 0 (mock) or 1x10⁴ (plaque-forming unit). As detailed below, several additional assessments were also completed each day, including body weight and body condition score (BCS). Criteria for humane endpoints were established in consultation with IACUC and collaborating veterinarians as described previously^{11 (link)}.
Discarded human specimens from COVID-19 patients were used to detect expression of ERK^1/2 phosphorylation. Use of human materials has been approved by Institutional Review Board (IRB).

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Xie J., Klemsz M.J., Kacena M.A., Sandusky G., Zhang X, & Kaplan M.H. (2022). Inhibition of MEK signaling prevents SARS-CoV2-induced lung damage and improves survival of infected mice. Journal of medical virology, 94(12), 6097-6102.

Publication 2022

Cg-Tg(K18-ACE2)2Prlmn/J mice SARS-CoV-2 isolate USA-WA1/2020

Ace2 Animal Body Body weight Covid 19 Erk1 Human Institutional animal care and use committee Mice Patients Phosphorylation Plaque Sars cov 2 Veterinarians Virus

Corresponding Organization : Indiana University – Purdue University Indianapolis

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Variable analysis

independent variables

Infection status (mock infected or SARS-CoV-2 infected)

dependent variables

Body weight
Body condition score (BCS)
ERK1/2 phosphorylation

control variables

Age of mice (approximately 8 weeks)
Viral concentration (0 or 1x10^4 plaque-forming unit)
Route of administration (intranasal, 20μl)

positive controls

None specified

negative controls

Mock infected group (media only)

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