Breast Cancer Cell Line Maintenance and Hormone Treatments

T47D cells were obtained from the American Type Culture Collection/National Cancer Institute (ATCC/NCI) Breast Cancer SPORE program, and MCF7 cells were purchased from the ATCC. Both cell lines were authenticated at the University of Arizona Genetics Core. T47D and MCF7 cells were maintained in RPMI 1640 medium + 10% FBS and DMEM + 5% FBS, respectively. For hormone treatment experiments, cells were deprived in phenol-red-free IMEM with 10% and 5% CSS for T47D and MCF7, respectively. CSS was purchased from Hyclone (#SH30068) and Gibco (#12676). 17β-estradiol (E2) and 4-hydroxytamoxifen (4OHT) were obtained from Sigma, and fulvestrant (Ful) and raloxifene were purchased from Tocris. AZD9496 recently described in Weir et al. [11 (link)] was kindly provided by AstraZeneca.