Assessing UCS Cell Viability

Viability of UCS cells was determined using the tetrazolium compound based CellTiter 96^® AQ_ueous One Solution Cell Proliferation (MTS) assay (Promega). Cells were plated in 96 well plate at density of 2.5x 10³ cells per well. 4h post serum starvation cells were subjected to TGFβ-I (R&D Biosystems) treatment alone or in presence of LY2157299 [40 (link)] (TGFβR-I inhibitor, Selleck Chem), LY2109761 [41 (link)] (TGFβR-I/II inhibitor, Selleck Chem), 10058-F4 (c-Myc Inhibitor, Sigma Aldrich), NFAT inhibitor (Cay-man Chemicals). After 24h treatment MTS assay was done and viability was calculated using vehicle as 100%. [42 (link)].

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Dhar Dwivedi S.K., McMeekin S.D., Slaughter K, & Bhattacharya R. (2015). Role of TGF-β signaling in uterine carcinosarcoma. Oncotarget, 6(16), 14646-14655.

Publication 2015

CellTiter 96® AQueous One Solution Cell Proliferation (MTS) assay 10058-F4 (c-Myc Inhibitor, NFAT inhibitor

Assay C myc Cell proliferation Cells Cells viability Ly2109761 Ly2157299 Promega Serum Tetrazolium Tgfβ i

Corresponding Organization :

Other organizations : University of Oklahoma Health Sciences Center

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Variable analysis

independent variables

TGFβ-I (R&D Biosystems) treatment
LY2157299 [40 (link)] (TGFβR-I inhibitor, Selleck Chem)
LY2109761 [41 (link)] (TGFβR-I/II inhibitor, Selleck Chem)
10058-F4 (c-Myc Inhibitor, Sigma Aldrich)
NFAT inhibitor (Cay-man Chemicals)

dependent variables

Viability of UCS cells

control variables

Cells were plated in 96 well plate at density of 2.5x 10^3 cells per well
Vehicle as 100% viability

positive controls

None specified

negative controls

Vehicle as 100% viability

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