Generation of Tamalin and Cytip Knockout Mice

Tamalin KO mice were kindly provided by Dr. Lino Tessarollo [58 (link)]. We obtained mice harboring the Cytip tm1a “knockout first” allele from the Mutant Mouse Resource and Research Centers (MMRRC) at University of California-Davis. The Cytip tm1a allele has loxP sites flanking exon 4 and 5. Mice heterozygous for Cytip tm1a were bred with mice harboring the Spo11-Cre transgene (C57BL/6-Tg Spo11-cre)Rsw/PecoJ), which express Cre recombinase in spermatocytes shortly after meiotic entry [77 (link)]https://sciwheel.com/work/citation?ids=1181041&pre=&suf=&sa=0&dbf=0. The resulting progeny from this cross harbored the Cytip tm1b KO allele. We subsequently bred mice harboring the Tamalin KO and Cytip KO alleles to create the Tamalin, Cytip DKO mice for analysis. We also bred mice heterozygous for Cytip tm1a allele with mice harboring FLP recombinase transgene (FLP tg/0) to produce progeny with the Cytip tm1c “conditional knockout” (cKO) allele. These mice were used to create the Cytip cKO mice that were homozygous for the Cytip tm1c allele and hemizygous for the Spo11-Cre transgene.