The concentration of sulfated glycosaminoglycan (GAG), elastin (ELN), collagen type 1 (COL 1), collagen type 2 (COL 2), and total hydroxyproline (HYP) content, in the auricular cartilage and engineered cartilage was assayed using commercially available ELISA kits (Jianglai Biotechnology, China) according to manual instruction. Absorbance was measured at 450 nm with a Microplate reader (PE, USA).
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