Quantitative ELISA for Biomarker Detection

We followed indirect and quantitative ELISA method.18 (link),19 (link) Plasma samples from RA, OA and HC were coated into 96-well microtiter plates (Nunc, USA), incubated with anti-TTR, anti-Serotransferrin, anti-ApoA1 and anti-RAGE antibodies (Santa Cruz, USA) separately followed by HRP-conjugated secondary antibody (Jackson, USA) incubation. For quantitative ELISA, commercialized available ELISA kit for TTR (Elabscience, E-EL-H2342), RAGE (Ray-Bio, ELH-RAGE) and ACPAs (EH4137) were used according to manufactures guideline (see details in supplementary methods).

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M.o.n.u., Agnihotri P., Saquib M., Sarkar A., Chakraborty D., Kumar U, & Biswas S. (2021). Transthyretin and Receptor for Advanced Glycation End Product’s Differential Levels Associated with the Pathogenesis of Rheumatoid Arthritis. Journal of Inflammation Research, 14, 5581-5596.

Publication 2021

96-well microtiter plates anti-ApoA1 anti-RAGE antibodies HRP-conjugated secondary antibody ELH-RAGE

Acpas Anti antibodies Antibody Apoa1 Elisa Plasma Rage Serotransferrin

Corresponding Organization : Institute of Genomics and Integrative Biology

Other organizations : Academy of Scientific and Innovative Research, All India Institute of Medical Sciences

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Variable analysis

independent variables

RA (rheumatoid arthritis) plasma samples
OA (osteoarthritis) plasma samples
HC (healthy control) plasma samples

dependent variables

TTR (transthyretin) levels
Serotransferrin levels
ApoA1 (apolipoprotein A1) levels
RAGE (receptor for advanced glycation end-products) levels
ACPA (anti-citrullinated protein antibody) levels

control variables

Coating of plasma samples into 96-well microtiter plates
Incubation with anti-TTR, anti-Serotransferrin, anti-ApoA1, and anti-RAGE antibodies
Incubation with HRP-conjugated secondary antibody
Use of commercialized ELISA kits for quantitative measurements of TTR, RAGE, and ACPAs

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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