Quantification of Arginine Metabolites

Plasma levels of l-arginine (the substrate for NO synthase), l-citrulline (the precursor of l-arginine), and asymmetric and symmetric dimethylarginine (ADMA and SDMA, both inhibitors of NO synthase) were determined according to our validated protocol [7 (link)]. We used a HPLC method (HP series 1100; Agilent Technologies Inc., Santa Clara, CA, USA) with fluorescence detection for OPA/3mercaptopropionic acid (3MPA) derivatives [7 (link)].

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Hsu C.N., Hou C.Y., Chang C.I, & Tain Y.L. (2021). Resveratrol Butyrate Ester Protects Adenine-Treated Rats against Hypertension and Kidney Disease by Regulating the Gut–Kidney Axis. Antioxidants, 11(1), 83.

Publication 2021

HP series 1100

Acid Arginine Derivatives Fluorescence Hplc Inhibitors L citrulline No synthase Plasma Symmetric dimethylarginine

Corresponding Organization : Chang Gung University

Other organizations : Kaohsiung Medical University, National Kaohsiung Marine University, National Kaohsiung University of Science and Technology

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Variable analysis

independent variables

L-arginine
L-citrulline
Asymmetric dimethylarginine (ADMA)
Symmetric dimethylarginine (SDMA)

dependent variables

Plasma levels of l-arginine
Plasma levels of l-citrulline
Plasma levels of ADMA
Plasma levels of SDMA

control variables

HPLC method (HP series 1100; Agilent Technologies Inc., Santa Clara, CA, USA) with fluorescence detection for OPA/3mercaptopropionic acid (3MPA) derivatives

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