Immunohistochemistry was performed as previously described 16 (link), 20 (link). Ki-67 primary antibody was purchased from Dako (Golstrup, Denmark). Tumor tissues were incubated with primary antibodies against Ki-67 and cleaved caspase-3, then incubated with a secondary antibody. Organ tissues were stained with H&E for the histological analysis.
Free full text: Click here