Luciferase Assay for lincRNA-p21 Promoter

Luciferase assays were performed as described in our previous study [22 (link)]. Briefly, HEK293T cells were cotransfected with each lincRNA-p21 promoter-luciferase construct and pRL-TK promoter Renilla luciferase construct in the vector group, wild-type p53 group and mutant p53 group for 48 h. Whole-cell lysates were extracted, and luciferase activity was determined using a dual luciferase reporter assay system (Beyotime, Shanghai, China) according to the manufacturer’s instructions. A pRL-TK promoter Renilla luciferase construct was used as an internal control.

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Jin S., Yang X., Li J., Yang W., Ma H, & Zhang Z. (2019). p53-targeted lincRNA-p21 acts as a tumor suppressor by inhibiting JAK2/STAT3 signaling pathways in head and neck squamous cell carcinoma. Molecular Cancer, 18, 38.

Publication 2019

dual luciferase reporter assay system

Assay Cell Lincrna Luciferase Renilla luciferase Vector

Corresponding Organization :

Other organizations : Shanghai Ninth People's Hospital, Shanghai Jiao Tong University

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Variable analysis

independent variables

LincRNA-p21 promoter-luciferase construct
P53 group (wild-type and mutant)

dependent variables

Luciferase activity

control variables

PRL-TK promoter Renilla luciferase construct (internal control)
HEK293T cells

controls

Vector group (negative control)
Wild-type p53 group
Mutant p53 group

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