Cre-Dependent Labeling of Somatostatin Neurons

SST-Ires-Cre mice (Ssttm2.1(cre)Zjh/J, Jackson Lab strain # 013044, [5] were crossed with Rosa-tdTomato reporter (B6;129S6-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J Jackson lab Strain # 007905, [18] (link)or GCAMP33 reporter (B6;129S-Gt(Rosa)26Sor/J Jackson lab Strain # 014538, mice, [17] (link). The resulting offspring displayed the Rosa-tdTomato or the GCAMP3 proteins, respectively, in the Cre-expressing neurons. Genotyping was performed with a commercial vendor (Transnetyx, Cordova, TN).

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Grosser S., Queenan B.N., Lalchandani R.R, & Vicini S. (2014). Hilar Somatostatin Interneurons Contribute to Synchronized GABA Activity in an In Vitro Epilepsy Model. PLoS ONE, 9(1), e86250.

Publication 2014

Ssttm2.1(cre)Zjh/J,

Ires Mice Neurons Proteins Rosa Strain Tdtomato

Corresponding Organization : Georgetown University

Other organizations : Charité - Universitätsmedizin Berlin

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Variable analysis

independent variables

Crossing SST-Ires-Cre mice with Rosa-tdTomato reporter mice or GCAMP33 reporter mice

dependent variables

Expression of Rosa-tdTomato or GCAMP3 proteins in the Cre-expressing neurons of the resulting offspring

control variables

Control variables not explicitly mentioned.

controls

Positive control: Not specified.
Negative control: Not specified.

Annotations

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