One of the viromes (MedDCM-Vir) was obtained from the DCM of the Mediterranean Sea (65 m deep) on August 29th, 2011. DNA was amplified by MDA and sequenced by Illumina to provide nearly 18 Gb of sequence data as was described in [24 (link)]. The other viromic sample (MetaVir-2013) was collected from the Mediterranean DCM at 55m (38.06851°N, 0.231994°W; bottom depth of 200 m) on 6 September 2013. Sample was processed in the same way as the other virome MedDCM-Vir [24 (link)]. However, the amount of DNA obtained was sufficient to sequence and it was not necessary to use any amplification treatment. Phages were concentrated using tangential flow filtration (TFF) with a 30 kD polyethersulfone membrane from Vivaflow (VF20P2). The resulting phage concentrate was ultracentrifuged (Optima XL 1000K Ultracentrifuge, Beckman) for 1 h at 4°C using a Type 70 Ti rotor (Beckman) at 30,000 rpm (92,600 g). The pellet was treated with 2.5 units DNase I at 37°C for 1 hr, and 70°C for 10 min to remove bacterial DNA. DNA was sequenced using Illumina Hiseq-2000 (100bp, paired-end read) (BGI, Hong Kong).
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