Protocol detail

Preparation and Maintenance of Bioactive Compounds

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Amphotericin B was purchased from Sigma-Aldrich (Madrid, Spain). Miltefosine was acquired from Cayman Chemicals (Vitro SA, Madrid, Spain). Staurosporine was isolated from the Streptomyces sanyensis PBLC04 strain (collected in Ecuador) (16 (link)). Laurinterol was obtained from the Laurencia johnstonii red algae, which was collected in Baja California, Mexico (17 (link)). Stock solutions of the compounds were prepared in dimethyl sulfoxide (DMSO) and maintained at −20°C until required.

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Arberas-Jiménez I., Rizo-Liendo A., Sifaoui I., Chao-Pellicer J., Piñero J.E, & Lorenzo-Morales J. (2022). A Fluorometric Assay for the In Vitro Evaluation of Activity against Naegleria fowleri Cysts. Microbiology Spectrum, 10(4), e00515-22.

Publication 2022

Amphotericin B Miltefosine

Amphotericin b Cayman Dimethyl sulfoxide Laurencia Laurinterol Miltefosine Red algae Staurosporine Strain Streptomyces sanyensis

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Variable analysis

independent variables

Amphotericin B
Miltefosine
Staurosporine
Laurinterol

dependent variables

Unspecified

control variables

Dimethyl sulfoxide (DMSO) was used to prepare stock solutions of the compounds
Stock solutions were maintained at -20°C until required

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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