Annexin V Apoptosis Assay Protocol

Test of cell apoptosis was via exerting Annexin V Kit (Beyotime, Beijing, China). Detachment of the cells was with 0.25% trypsin, removal of digestive juice was conducted, and placing of the cells was in the previously collected medium. Centrifugation at 12,000 RPM and abandonment of the supernatant were performed. Collection of the cell precipitates and production of the cell suspension with phosphate buffer saline were conducted. Centrifugation again and removal of the supernatant were conducted; Addition of Annexin V fluorescein-isothiocyanate and 300 mL propidium iodide labeling solution and incubation were performed [7 (link)].