EdU staining was done using Click-iT™ EdU Cell Proliferation Imaging Kit (ThermoFisher Scientific) according to manufacturer’s instructions. Annexin V/DAPI staining was done as previously described68 (link),69 (link). For assessing the effects of SOC on G1 arrest, T47D cells treated with 5 µM of SOC for 24 hours were fixed in ethanol for 30 min on ice and stained with DAPI. For analyzing cell cycle distribution upon SOC treatment at early time points, BrdU/7AAD Flow kit (BD Biosciences) was utilized based on manufacturer’s instructions.
Saatci O., Cetin M., Uner M., Tokat U.M., Chatzistamou I., Ersan P.G., Montaudon E., Akyol A., Aksoy S., Uner A., Marangoni E., Sajish M, & Sahin O. (2023). Toxic PARP trapping upon cAMP-induced DNA damage reinstates the efficacy of endocrine therapy and CDK4/6 inhibitors in treatment-refractory ER+ breast cancer. Nature Communications, 14, 6997.
Other organizations :
MUSC Hollings Cancer Center, Medical University of South Carolina, Hacettepe University, Bilkent University, University of South Carolina, Université Paris Sciences et Lettres, Institut Curie, Hacettepe University Hospital
Cell cycle distribution (DAPI staining, BrdU/7AAD)
control variables
Cell lines (T47D)
controls
Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.
Annotations
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