Selective HDAC Inhibitors for Lung Allograft Transplant

Valproic acid for selective HDAC1 inhibition, RGFP966 for selective HDAC3 inhibition, LMK-235 for selective HDAC4 inhibition, Tubastatin A for selective HDAC6 inhibition, PCI-34051 for selective HDAC8 inhibition, and TSA for pan-HDAC inhibition were purchased from Selleckchem (Houston, TX, USA) and were used in various treatment groups. The chemicals were dissolved in dimethyl sulfoxide (DMSO; Sigma-Aldrich, St. Louis, MO, USA). Vehicle (DMSO) was used in control groups. 2 d before lung allograft transplant, the lung allograft recipient mice were treated with the vehicle, Valproic acid (300 mg/kg) 70 (link),71 (link), RGFP966 (10 mg/kg) 72 (link),73 (link), LMK-235 (20 mg/kg) 74 (link), Tubastatin A (30 mg/kg) 75 (link), PCI-34051 (40 mg/kg) 76 (link), or TSA (1 mg/kg) 77 (link) by intraperitoneal injection every day until lung allograft loss which was determined by Micro-Computer Tomography (micro-CT) Scans, unless specified.

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Zhou W., Yang J., Saren G., Zhao H., Cao K., Fu S., Pan X., Zhang H., Wang A, & Chen X. (2020). HDAC6-specific inhibitor suppresses Th17 cell function via the HIF-1α pathway in acute lung allograft rejection in mice. Theranostics, 10(15), 6790-6805.

Publication 2020

Valproic acid RGFP966 LMK-235 Tubastatin A PCI-34051 DMSO

Allograft Dmso Inhibition Intraperitoneal injection Lmk 235 Lung Lung transplant Mice Pci 34051 Rgfp966 Scans Tomography Tsa 1 Valproic acid

Corresponding Organization :

Other organizations : Shanghai Jiao Tong University, Shanghai Chest Hospital, Fudan University, Huashan Hospital

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Variable analysis

independent variables

Valproic acid (300 mg/kg)
RGFP966 (10 mg/kg)
LMK-235 (20 mg/kg)
Tubastatin A (30 mg/kg)
PCI-34051 (40 mg/kg)
TSA (1 mg/kg)

dependent variables

Lung allograft loss determined by Micro-Computer Tomography (micro-CT) Scans

control variables

Vehicle (DMSO)

controls

Vehicle (DMSO)
None specified

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