To detect cytokines, BAL fluid and sera were collected and stored at −80 °C. IL-1β was quantified by ELISA according to manufacturer’s instructions (Becton Dickinson). Levels of IL-18 were determined by ELISA as previously described34 (link). Levels of IL-6, CCL2, IFNβ, IL-10, IL-12p70, CCL5, CXCL1 and TNFα proteins were determined by cytokine bead array, mouse inflammation and flex kit (Becton Dickinson).
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