Adipogenic Tissue-Derived MSC Characterization

For all experiments, human adipogenic tissue-derived mesenchymal stem cells (MSCs) were used. After obtaining the donor’s informed written consent, as approved by the Institutional Review Board (Hannover Medical School) with the reference number 3475-2017, adipose tissue was received following abdominoplasty surgery. After isolation, MSCs have been extensively characterized by surface marker analysis and functional properties as described earlier [26 (link)]. Cultivation of MSCs was performed in cell culture medium in a 5% CO₂, 21% O₂, humidified atmosphere at 37 °C (Heracell 150i incubator, Thermo Fisher Scientific Inc., Waltham, USA). The MSCs were routinely maintained in 75 cm² cell culture flasks (Corning, CellBind Surface, Corning, NY, USA), and then harvested at about 85% confluency by accutase treatment (Merck KGaA, Darmstadt, Germany) for detachment [26 (link)]. 24 h prior to the start of an experiment, cells were seeded in 6-, and 96-well plates (at a density of 18,000 cells·cm⁻² and 1100 cells·cm⁻², respectively) (Sarstedt AG and Co. KG, Nürnbrecht, Germany). Experiments were performed with cells of passages two to six.