Salmonella Infection in Macrophage Model
Corresponding Organization : National Institutes of Health
Protocol cited in 16 other protocols
Variable analysis
- Phorbol 12-myristiate-12 acetate (PMA) treatment
- Presence of macrophage colony-stimulating factor (MCSF)
- Infection of THP-1 cells and primary human monocytes with Salmonella enterica serovar Typhimurium SL1344 wild type and mCherry-expressing strains
- Cell culture conditions (RPMI + Glutamax medium, 10% FBS, humidified 37°C, 5% CO2 incubator)
- Use of low passage (≤15) THP-1 cells
- Isolation of primary human monocytes using negative selection
- Use of 8-chamber Ibidi dishes or 24-well tissue culture treated plates for cell plating
- Not explicitly mentioned
- Not explicitly mentioned
Annotations
Based on most similar protocols
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!