Radiolabeled Lipid Nanoparticle Synthesis

1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), cholesterol (chol), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-mPEG2000), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide(polyethylene glycol)-2000] (DSPE-PEG2000-Mal), 1,2-distearoyl-snglycero-3-phosphoethanolamine-N-[amino(-polyethyleneglycol)-2000] (DSPE-PEG2000-NH₂), and 1,2-dioleoyl-snglycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (DOPE-rho) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). 2-S-(4-isothiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic acid was purchased (p-SCN-Bn-NOTA) from Macrocyclics (Dallas, TX, USA). Copper-64 was produced at the Korea Institute of Radiological and Medical Sciences (KIRAMS, Seoul, Republic of Korea) by 50-MeV cyclotron irradiation [56 (link)]. Chloroform, methanol, dimethylformamide (DMF), and triethylamine (TEA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Cetuximab was purchased from Merck KGaA (Darmstadt, Germany). Amicon filters were purchased from Millipore (Billerica, MA, USA).

Free full text: Click here

Jeong H.Y., Kang S.J., Kim M.W., Jeong I.H., Choi M.J., Jung C., Song I.H., Lee T.S, & Park Y.S. (2024). Development of PET Radioisotope Copper-64-Labeled Theranostic Immunoliposomes for EGFR Overexpressing Cancer-Targeted Therapy and Imaging. International Journal of Molecular Sciences, 25(3), 1813.

Publication 2024

1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine DSPE-mPEG2000 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N 1,2-dioleoyl-snglycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (DOPE-rho) p-SCN-Bn-NOTA Chloroform methanol dimethylformamide (DMF), triethylamine (TEA) Cetuximab Amicon filters

Corresponding Organization : Yonsei University

Other organizations : Korea University, Korea Institute of Radiological and Medical Sciences

Top 5 similar protocols

Variable analysis

independent variables

1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC)
Cholesterol (chol)
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-mPEG2000)
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide(polyethylene glycol)-2000] (DSPE-PEG2000-Mal)
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(-polyethyleneglycol)-2000] (DSPE-PEG2000-NH2)
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (DOPE-rho)
2-S-(4-isothiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic acid (p-SCN-Bn-NOTA)
Copper-64
Cetuximab

dependent variables

Not explicitly mentioned

control variables

Chloroform
Methanol
Dimethylformamide (DMF)
Triethylamine (TEA)
Amicon filters

positive controls

Not specified

negative controls

Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!