IHC analysis was performed as previously described.
17 (link) Tissue sections were incubated with polyclonal rabbit anti‐Siglec‐15 antibody (abcam, ab198684, 1:150) in TBS. Siglec‐15 immunostaining score was examined by two autonomous pathologists on the basis of intensity and percentage of positive staining cells. The detailed protocol was described in our previous studies.
19 (link),
20 (link) Briefly, the degree of Siglec‐15 staining was defined as follows: Samples with a final score <4 were recognized as low expression while those with a final score ≥4 were determined as high expression. Samples with a final score = 0 were classified as negative expression.
17 (link) Tissue sections were incubated with polyclonal rabbit anti‐Siglec‐15 antibody (abcam, ab198684, 1:150) in TBS. Siglec‐15 immunostaining score was examined by two autonomous pathologists on the basis of intensity and percentage of positive staining cells. The detailed protocol was described in our previous studies.
19 (link),
20 (link) Briefly, the degree of Siglec‐15 staining was defined as follows: Samples with a final score <4 were recognized as low expression while those with a final score ≥4 were determined as high expression. Samples with a final score = 0 were classified as negative expression.
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