Cardiac Enzymes and Cytokine Profiling

ELISA kits were consumed to measure cardiac enzymes; CPK (ab187396), CK-MB (ab187396), cTnT (ab246529) and cTnI (ab246529) (all obtained from Abcam, Eugene, OR, USA), following the manufacturer’s protocols [45 (link)]. The concentration of serum cytokines TNF-α, IL-17, IL-10, and IL-6 was determined using ELISA kits (R&D Systems; Minneapolis, MN, USA). Serum IL-2 and IL-4 were determined by assay kits (Eagle Biosciences, Inc., Amherst, NH, USA).

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Marino Y., Arangia A., D’Amico R., Cordaro M., Siracusa R., Impellizzeri D., Gugliandolo E., Fusco R., Cuzzocrea S, & Di Paola R. (2023). Aggravation of TGFβ1-Smad Pathway and Autoimmune Myocarditis by Fungicide (Tebuconazole) Exposure. International Journal of Molecular Sciences, 24(14), 11510.

Publication 2023

ab187396 ELISA kits

Assay Cardiac Ck mb Cytokines Eagle Elisa Enzymes Il 10 Il 17 Serum Tnf α

Corresponding Organization : University of Messina

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Variable analysis

independent variables

ELISA kits used to measure cardiac enzymes: CPK (ab187396), CK-MB (ab187396), cTnT (ab246529) and cTnI (ab246529)
ELISA kits used to measure serum cytokines: TNF-α, IL-17, IL-10, and IL-6 (R&D Systems)
Assay kits used to measure serum IL-2 and IL-4 (Eagle Biosciences, Inc.)

dependent variables

Concentration of cardiac enzymes: CPK, CK-MB, cTnT and cTnI
Concentration of serum cytokines: TNF-α, IL-17, IL-10, and IL-6
Concentration of serum IL-2 and IL-4

control variables

Manufacturer's protocols followed for ELISA and assay kits

controls

No positive or negative controls explicitly mentioned

Annotations

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