For IHC, 4 μm thick sections were cut from paraffin-embedded blocks and dried onto positively charged microscope slides. Slides were prepared and stained using the ImmPRESS® HRP Anti-Mouse IgG (Peroxidase) Polymer Detection Kit (Vector Laboratories) as previously described [23 (link)].
For analysis, tissue sections were imaged using an EVOS M7000 automated microscope (Thermo Fisher Scientific). Images were de-identified and 50 random fields per section were run through QuPath image analysis software [24 (link)] to quantify positive DAB-stained cells (as a percentage of total cells).
For analysis, tissue sections were imaged using an EVOS M7000 automated microscope (Thermo Fisher Scientific). Images were de-identified and 50 random fields per section were run through QuPath image analysis software [24 (link)] to quantify positive DAB-stained cells (as a percentage of total cells).
