Apoptosis detection protocol

Apoptosis detection was performed and counted 48 hours after treatments as described (2 (link),3 (link),30 (link),31 (link)). Earlier apoptosis was detected with caspase-3/7 CellEVENT (Thermo).

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Garcia-Rendueles A.R., Chenlo M., Oroz-Gonjar F., Solomou A., Mistry A., Barry S., Gaston-Massuet C., Garcia-Lavandeira M., Perez-Romero S., Suarez-Fariña M., Pradilla-Dieste A., Dieguez C., Mehlen P., Korbonits M, & Alvarez C.V. (2021). RET signalling provides tumorigenic mechanism and tissue specificity for AIP-related somatotrophinomas. Oncogene, 40(45), 6354-6368.

Publication 2021

CellEVENT

After treatments Apoptosis Caspase 3

Corresponding Organization :

Other organizations : Center for Research in Molecular Medicine and Chronic Diseases, Universidade de Santiago de Compostela, Queen Mary University of London, William Harvey Research Institute, Université Claude Bernard Lyon 1, Centre National de la Recherche Scientifique, Centre Léon Bérard, Inserm, Centre de Recherche en Cancérologie de Lyon

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Variable analysis

independent variables

Treatments
Time of apoptosis detection (48 hours after treatments)

dependent variables

Apoptosis detection and counting

control variables

Apoptosis detection method (caspase-3/7 CellEVENT (Thermo))
Time of earlier apoptosis detection

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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